Fig. 6: Determination of escape frequencies of O-methyl-L-tyrosine (OMeY)-dependent, engineered Bacillus subtilis. | Nature Communications

Fig. 6: Determination of escape frequencies of O-methyl-L-tyrosine (OMeY)-dependent, engineered Bacillus subtilis.

From: Titrating bacterial growth and chemical biosynthesis for efficient N-acetylglucosamine and N-acetylneuraminic acid bioproduction

Fig. 6

a After 15 days of cultivation, an escapee appeared on OMeY-free plates. b Escape frequencies of three OMeY-dependent B. subtilis strains were determined by plate coating. The escape efficiencies of all three strains on day 1 were under the assay limit, which is not shown in the plot. The data are expressed as the mean ± SEM from three (n = 3) biologically independent replicates. Source data are provided as a Source data file. c Scanning electron microscopic morphological identification of four engineered strains in the presence or absence of OMeY. Scanning electron microscopy experiments were performed three times independently with similar results. Source data underlying b are provided as a Source data file.

Back to article page