Fig. 3: Quantification of RNA isoforms with alternative TSSs and TESs. | Nature Communications

Fig. 3: Quantification of RNA isoforms with alternative TSSs and TESs.

From: Single-cell RNA cap and tail sequencing (scRCAT-seq) reveals subtype-specific isoforms differing in transcript demarcation

Fig. 3

a Scatterplot of observed transcript expression levels (y-axis) and true abundance (x-axis) of ERCC spike-ins through 5′-end quantification (n = 92). Each point represents a transcript. The Pearson’s correlation coefficient is shown in the upper right corner. b Scatterplot shows the Pearson’s correlation of transcriptional level of isoforms between replicated pools of three single cells. c Heatmap for Pearson’s correlation coefficient of transcriptomes of DRG neuron and oocytes, based on 5′-end quantification of RNA isoforms. d Heatmap showing RNA isoforms of alternative TSS choices with cell-type specificity. The major isoforms either in oocytes or in DRG neurons are shown (n = 372 isoforms). e Genome browser tracks showing the alternative choices of TSS of Tse22d1 between oocytes and DRG neurons. f Squared coefficients of variation of scRCAT-seq (n = 4) and ScISOr-seq (n = 4), versus the means of normalized read counts. Shown is the mean of squared coefficients of variation shaded by 95% confidence intervals. Source data are provided as a Source data file.

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