Fig. 8: Mice with global S20E KI are protected against myocardial infarction. | Nature Communications

Fig. 8: Mice with global S20E KI are protected against myocardial infarction.

From: CHIP phosphorylation by protein kinase G enhances protein quality control and attenuates cardiac ischemic injury

Fig. 8

a Guide RNA to target CHIP S20/S24 region, and the replacement RNA that includes a silent mutation of the PAM sequence to further enhance homology directed repair. b CHIPS20E (n = 10 heterozygous; n = 7 homozygous) mice have reduced mortality following myocardial infarction due to total coronary occlusion. c Example M-mode echocardiograms show improved wall motion and less heart dilation in CHIPS20E (SE) KI mice versus littermate (WT) controls. d Summary data for LV function and volumes from same experiment. N = 5 mice for each group, mean ± SEM, p-value shown for Mann–Whitney test unpaired test. e Whole heart cross-section histology shows larger infarct territory in WT versus CHIPS20E hearts. Summary quantitation (right). N = 5 biological replicates, mean ± SD, p value for Mann–Whitney unpaired test. Source data are provided as a Source Data file.

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