Fig. 3: Hyperbranching is induced by specific diol oxylipins.

a Hyphal-branching analysis of Af293 WT when 8R-HODE (5 µg/mL) alone, 5,8-diHODE (5 µg/mL) alone, and 8R-HODE and 5,8-diHOE (both at 5 µg/mL) were treated to spores and grown for 20 h in microfluidic wells (n = 6 for no oxylipin, 8R-HODE alone, 5,8-diHODE, and n = 5 for 5,8-diHODE + 8R-HODE). b Branching analysis when GR24, a synthetic strigolactone, was treated to Af293 WT at 50 or 5 µg/mL compared to the acetone solvent control (n = 6). c Screen of a panel of dihydroxy oxylipins on Af293 WT for hyphal branching (n = 6). For all experiments, six hyphae were randomly selected across microfluidic wells and imaged every 15 min between 20 h and 25 h post inoculation. Compounds were either commercially purchased or purified in the laboratory and resuspended in EtOH. Brown–Forsythe and Welch ANOVA tests were performed, followed by Dunnett’s T3 multiple comparison test in between each group in (a), and between the solvent control and each treatment group in (b, c). All values represent mean ± SEM. P values corresponding to asterisks from top to bottom in (c) are: 0.0372, 0.0146, 0.0001. ns not significant (P > 0.05).