Fig. 4: Branching response of several filamentous fungal species that contain a PpoA ortholog.

a Conidia of tested fungal species were inoculated in either GMM containing 1% EtOH or 5 µg/mL 5,8-diHODE and incubated in 96-well plates (n = 6). Six germinated hyphae were imaged via time-lapse microscopy for each treatment in each strain, and branching was assessed at specific time points when both apical and lateral growth were evident. All cultures were incubated at the optimal and permissive temperature specific to the species, and branching quantification was performed at a stage with pronounced apical growth and a significant degree of lateral branching. Multiple two-sided t tests were used to compare between treatment groups in each fungus. b Images of M. grisea cultures after exposure to either EtOH (1%) or 5 µg/mL 5,8-diHODE for 16 h. Images are representative of six microscopic images acquired for each condition. Scale bar represents 20 µm. All values represent mean ± SEM. ns not significant (P > 0.05). A   Aspergillus, P   Penicillium, B   Botrytis, M   Magnaporthe.