Fig. 1: Experimental design and QC of multi-omics datasets of murine in vitro neuronal differentiation. | Nature Communications

Fig. 1: Experimental design and QC of multi-omics datasets of murine in vitro neuronal differentiation.

From: DOT1L-mediated murine neuronal differentiation associates with H3K79me2 accumulation and preserves SOX2-enhancer accessibility

Fig. 1

a Experimental design of this study. We differentiate mESC towards neural progenitors (NPC48h) in vitro. We treat mESC and NPC for 48 h either with the DOT1L inhibitor EPZ5676 (10 μM) or with DMSO (1/1000) as control. For each sample, we generate transcriptomics data via RNA-seq and comprehensive epigenomes using quantitative ChIP-seq. We further map the accessible chromatin landscape for NPC48h using ATAC-seq. b Upper panel: principal component analysis of the transcriptome of mESC and NPC48h on the top 500 most variable genes (rlog transformed counts) shows a separation between the two cell types on the first principal component. Lower panel: multiple factor analysis of the epigenome of mESC and NPC48h computed over the top 500 most variable 2 kb windows for each histone modification yield similar results. Biological replicates are denoted by the same color.

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