Fig. 2: PCOS mice gain excess weight regardless of macronutrient balance.
From: Defining the impact of dietary macronutrient balance on PCOS traits
a Body weight; regardless of diet PCOS mice exhibit increased body weight (P < 0.001). b Body fat (g), calculated by dual-energy x-ray absorptiometry (DEXA), showing that PCOS mice display a significant increase in body fat (P = 0.0005). c Fat depot weights showing a significant increase in adiposity in PCOS mice (P < 0.05). d Lean body mass (g) calculated by DEXA, showing that PCOS mice exhibit a significant increase in lean body mass (P < 0.001). a–d Significance determined by Mann–Whitney test, asterisk (*) indicates P-value <0.05. e Representative DEXA images of a control and PCOS mouse, showing development of increased adiposity in PCOS mouse. f–k 3D GAM response surfaces showing the relationship between macronutrient intake (kJ/day) and body weight in control (f–h) and PCOS (i–k) mice. Red areas indicate the greatest value, which decreases to the lowest value as the color shifts to blue. Response surfaces show that dietary macronutrient intake influenced body weight similarly in control and PCOS mice, as color shifts from blue to red along the P, C, and F axis. Direct comparison of control and PCOS mice on <10 kJ/day P (l), n = 56 control and 62 PCOS mice, <20 kJ/day C (m), n = 74 control and 68 PCOS mice and <20 kJ/day F intakes (n), n = 76 control and 70 PCOS mice, demonstrate that PCOS mice are significantly heavier than control mice on similar P, C, and F intakes (P < 0.001). o–q 3D GAM response surfaces showing the effects of C and F intakes on body weight in control mice (o), PCOS mice (p) and the response difference (q), revealing PCOS mice exhibited a significantly different (higher) body weight compared to control mice at C and F intake <25 kJ/day. Above a C and F intake of 25 kJ/day, PCOS mice exhibit a comparable body weight response to macronutrient intake as controls. a–d, f–k, o–q, n = 93 control and 94 PCOS mice. a–d, l–n Data presented as violin plots, doted lines indicate the median and dashed lines the 25 and 75% percentiles.
