Fig. 2: Cloning of qNLA1/qCel1.
From: MYB61 is regulated by GRF4 and promotes nitrogen utilization and biomass production in rice
a QTL analyses of nitrogen-mediated leaf area changes (qNLA, black line) and cellulose content (qCel, purple line) in the 9311 introgression lines with NP background. The green lines were used to separate individual chromosomes. b qNLA1 is colocalized with qCel1. Different symbols indicate the positions where the corresponding QTL are located. The numbers on the left side of chromosome indicate physical locations (Mb). c Mapping of qNLA1 and qCel1 in the backcross population. The numbers on chromosome 1 indicate the physical locations. S1–S5 and M1–M7 indicate molecular markers used for mapping. The black and white rectangles represent 9311 and NP genomic region, respectively. Arrows represent candidate genes within the 231.8 kb pin-pointed region according to the performance of the CSSL recombinants (1 to 5) shown in Supplementary Fig. 2f, g. d The expression differential ratio of the candidate genes in the NIL to NP plants. Rice HNR was used for normalization of the expression of the examined genes. Error bars represent the mean ± SD of three biological replicates. e The variation at Os01g18240 gene. A helitron element is present in the promoter of the NP allele. Source data underlying Fig. 2d are provided as a Source Data file.
