Fig. 1: Expression of four L. japonicus miR2111 genes, including those identified by RNA-seq analysis. | Nature Communications

Fig. 1: Expression of four L. japonicus miR2111 genes, including those identified by RNA-seq analysis.

From: MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus

Fig. 1

a–d RNA-seq read coverage of MIR2111-2 (a), MIR2111-4 (b), MIR2111-5 (c), and MIR2111-7 (d) were visualized by the Integrative Genomics Viewer. All data were acquired by RNA-seq of mature leaves of wild-type (MG-20) and har1-7 plants that were inoculated with M. loti (1 day after inoculation) or mock-treated (control). RNA-seq libraries were prepared using poly(A) enrichment method, and miR2111 genes were predicted by RNA-Seq-based gene prediction using Stringtie version 1.3.4d with default settings. Read abundance normalized in bins per million (BPM) is shown. Gray arrows in the gene column represent the directions of mature miR2111 (Bottom). Regions used for the over-expression assay are indicated as black two-directional arrows (Top) (see Fig. 2). e Transcripts per million (TPM) values of four miR2111 genes. Scatterplots show individual biological replicates as dots. Bars indicate mean values.

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