Fig. 5: MIR2111-5 knockout significantly inhibited nodulation and accumulation of mature miR2111s. | Nature Communications

Fig. 5: MIR2111-5 knockout significantly inhibited nodulation and accumulation of mature miR2111s.

From: MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus

Fig. 5

a Nucleotide sequences of MIR2111-5 and its knockout lines. b The numbers of nodules (21 days after inoculation) and infection threads (5 and 7 days after inoculation) on roots of WT, mir2111-5-1, and mir2111-5-2. Bars indicate mean ± standard deviation. c qRT-PCR analyses of mature miR2111s, MIR2111-5, and TML in leaves and roots at 14 and 7 days after germination, respectively. n = 3 individual biological replicates for each treatment. Bars indicate mean values. b, c Scatterplots show individual biological replicates as dots. Different letters indicate significant differences (P < 0.05) from Tukey’s honestly significant difference test.

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