Fig. 2: Shh maintains Siah2 expression in a Ras/Mapk-dependent manner.
a Siah2 transcript expression in the different subgroups of human medulloblastomas. U uncategorized (the subset of tumors that do not belong to any of the four subgroups). Data obtained from the Pediatric Cancer Genome Project (https://pecan.stjude.cloud/proteinpaint/SIAH2). Boxplots represents the median, interquartile range, maximum and mininum. b Representative immunofluorescent staining of Siah2 on n = 3 independent mouse Ptc+/− P18KO medulloblastoma tumors. Dotted lines demarcate the boundary between normal and transformed tissue. Light blue scale bar = 100 μm. c–e Isolated GNPs from P7 WT cerebella were cultured on Matrigel-coated glass as indicated. cm conditioned medium. c Cells were fixed after 24 and 48 h followed by Siah2 immunofluorescence staining. Quantification shows the mean percentage of Siah2-positive cells. d, e Cells were co-nucleofected with the nuclear marker H2B-mCherry and the indicated constructs and cultured for 48 h followed by fixation and immunofluorescence for Siah2. Siah2 expression was measured using SlideBook. Quantification shows mean intensity measurements (d) or percent positive (e). All quantifications c–e represent 3 biological replicates with n = 18 confocal image fields analyzed. P values represent unpaired one-tailed student’s t test performed when compared to LacZ (d) or as indicated. Scale bar = 10 μm. Error bars represents mean ± SD.
