Fig. 3: Shh signaling blocks GZ exit in a Ras/Mapk and Siah2-dependent manner.

a Ex vivo cerebellar pulse-chase experiment schema using P7 Ptch1^Flox/Flox (Ptc^F/F) cerebella for panels b and c. b Post fixation, the cerebellar slices were processed for immunofluorescent staining of Siah2. Quantification shows mean Siah2-positive cells in H2B-mCherry electroporated cells (Cre^Inactive + Erk shRNA, n = 28; all other conditions, n = 31, confocal image fields sampled from 3 independent experiments on 3 different cerebella). c Post fixation, the cerebellar slices were stained for EdU using the Invitrogen Click-it Edu staining kit according to the manufacturer’s instructions. Quantification of 4 independent experiments show the mean frequency distribution within and outside the EGL (top panel), the average migration distance (middle panel), and the percentage of electroporated cells that were EdU positive (lower panel), which accounts for at least n > 8000 individual cells counted in every condition. Horizontal length of representative images represent radial distance of 300 μm from cerebella pial. P values represents unpaired one-tailed student’s t test when compared to Cre^Active + Control shRNA. Scale bar = 10 μm. Error bars represents mean ± SD.