Fig. 2: SARS-CoV-2 infection induces general lymphopenia and CD8, VD1 and VD2 activation. | Nature Communications

Fig. 2: SARS-CoV-2 infection induces general lymphopenia and CD8, VD1 and VD2 activation.

From: Whole blood immunophenotyping uncovers immature neutrophil-to-VD2 T-cell ratio as an early marker for severe COVID-19

Fig. 2

a Absolute counts of T-cell compartments in healthy donors, acute and recovered COVID-19 patient. b UMAP clustering of CD3+ cells. c left panel: CD45RA and CD27 gating strategy example on CD8+ T-cells; right panel: heatmap representation of mean frequencies of T-cell differentiation across the groups, individual plots given in Supplementary Fig. 2. d Changes in CD38 gMFI in naïve, CM, EM and TEMRA for CD8, CD4, VD1 and VD2 T-cells. ND indicates not determined since frequency of these compartment was too low for accurate gMFI measurement. Data presented are from individual human samples of healthy n = 19, acute n = 54 and recovered n = 28 from flow panel B. Absolute counts and frequency were analysed by Kruskal–Wallis using Dunn correction for multiple comparison, gMFI was analysed by Brown–Forsythe and Welch ANOVA using Dunnett T3 correction for multiple comparison. Scatter dot plots are presented as mean ± SD. For heatmaps, stars shown in acute column represent healthy vs acute comparison. Stars shown in recovered column represent acute vs recovered comparison. *p < 0.05, **p < 0.01, ***p < 0.001. Data available in source data file, exact p-values are given in Supplementary Data 1.

Back to article page