Fig. 5: Stress granules are formed during glucose starvation.
From: ATP hydrolysis by yeast Hsp104 determines protein aggregate dissolution and size in vivo
a On average, 22% of cells had an Rnq1-mCherry aggregate during glucose starvation. b In hsp42Δ background, the abundance of Hsp104-GFP containing aggregates is reduced to ~20% compared to the WT strain. Rnq1-mCherry aggregates are present in ~25% of cells during glucose starvation. c Hsp42-GFP aggregates are present in ~50% and ~75% of cells at 0.2% and 0.02% glucose, respectively. d In WT background, ~25% of cells display an Nrp1-mCherry-tagged compartment during glucose starvation. e In hsp42Δ background, an average of 25% of cells remain with Hsp104-GFP aggregates and ~22% with Nrp1-mCherry puncta during glucose starvation. For all panels, n = 1000 cells were screened for aggregates starting from three independent exponential yeast cultures for each condition. Bar height represents mean ± SD from three separate cultures, each performed in triplicate. The mean of three technical replicates for each biological replicate is displayed as single data points. ***p < 0.001; **p < 0.01; *p < 0.05 (one-way ANOVA plus Tukey post hoc). The images are representative of three biological replicates, each in three technical replicates. The black bar represents 8 µm.
