Fig. 7: Protein compartmentalization during metabolic stress confers fitness advantage.

a Scheme of the competition assay. In the mid-exponential phase the cells were pelleted, and then resuspended in YPD medium with 0.2% and 0.02% glucose for 90 min. Equal representation of the cells was ensured. This was followed by plating the serial dilutions on YPD-agar plates without kanamycin, and dilutions with between 50 and 200 colonies were replica plated on YPD-agar plates with kanamycin (50 µg/mL). Colonies were counted on both types of plates, and the ratio of kanamycin-resistant (mutant) to kanamycin-sensitive (WT) colonies was calculated. Following the same centrifugation procedure, the cells were transferred back into YPD medium with 2% glucose and grown for 24 h counting from the moment of initial dilution. In parallel, a series of the same samples was continuously kept in 2% glucose for control. The same treatment was applied to the control cells to take into account the potential effects of the centrifugation on cell survival. KanS stands for a kanamycin-sensitive phenotype; KanR stands for a kanamycin-resistant phenotype. b Competitive fitness of the WT strain is unaffected by the kanamycin-resistant phenotype in optimal conditions, and during the starvation. c Competitive fitness of yeast cells is strongly decreased in the hsp42Δ and Hsp104^E687Q mutant in both 0.2% and 0.02% glucose. Bar height represents mean ± SD from six separate cultures, each performed in triplicate. The mean of three technical replicates for six biological replicates is displayed as single data points. ***p < 0.001; **p < 0.01; *p < 0.05 (one-way ANOVA plus Tukey post hoc).