Fig. 2: Oxidized and therapeutic dNTPs inhibit telomerase processivity.
From: Mechanisms of telomerase inhibition by oxidized and therapeutic dNTPs
Telomerase reactions were conducted with (TTAGGG)3 primer and cellular-concentration dNTPs except that the indicated natural or modified dNTP was titrated in at increasing concentrations (0, 5, 125 μM) along with either 0.3 μM [α-32P]dTTP a or 0.3 μM [α-32P]dGTP b, c to label the products. The loading control (*) was a 32P-end labeled 18-mer oligonucleotide. Numbers on the left indicate the number of added repeats; letters on the right indicate template residue. Images are representative of three independent experiments.
