Fig. 3: Telomerase insertion of modified dNTPs.
From: Mechanisms of telomerase inhibition by oxidized and therapeutic dNTPs
Telomerase reactions were conducted with 5 nM 32P-end labeled primer a Primer 1 (TTAGGG)3, b Primer 2 (GGTTAG)3, or c Primer 3 (AGGGTT)3. Reactions contained cellular-concentration dNTPs (lane 1) or 50 μM of indicated natural dNTP (blue, lanes 2–5), oxidized dNTP (red, lanes 6–8), or therapeutic dNTP (green, lanes 9–11). Products were separated on denaturing gels. 8dG (8-oxo-dGTP); 8dA (8-oxo-dATP); 2dA (2-OH-dATP); 6dG (6-thio-dGTP); ddI (ddITP); ZdT (AZT-TP). Letters on the right indicate the template base; P indicates unextended 18-mer primer. Graphs represent the percent of total primers extended. Images are representative of, and data are shown as mean ± s.d. from, three independent experiments. Source data are provided as a Source Data file.
