Fig. 5: POT1–TPP1 fail to overcome inhibitory dNTPs.
From: Mechanisms of telomerase inhibition by oxidized and therapeutic dNTPs
Direct telomerase assays were conducted in the absence or presence of 500 nM POT1 and 500 nM TPP1, as indicated, and 5 nM 32P-end labeled primer (TTAGGGTTAGCGTTAGGG) designed to position POT1 at the 10 nt primer 5′ end. Reactions contained cellular-concentration dNTPs (lanes 1 and 2), except when the natural dNTP was substituted with the oxidized dNTP a or therapeutic dNTP b analog (lanes 3–8), as indicated. 8dG (8-oxo-dGTP); 8dA (8-oxo-dATP); 2dA (2-OH-dATP); 6dG (6-thio-dGTP); ddI (ddITP); ZdT (AZT-TP). Letters on the right indicate template base; P indicates unextended 18-mer primer. c Processivity was calculated on the basis of total products normalized to loading control. Images are representative of, and data are shown as mean ± s.d. from 3 to 5 independent experiments. Source data are provided as a Source Data file.
