Fig. 2: Frequent i-loops in telomere-enriched samples.
From: Telomere damage induces internal loops that generate telomeric circles
a Examples of molecules with i-loops found in the telomeric samples. Telomeric DNA, enriched with the procedure described in Fig. 1, was analyzed in transmission Electron Microscopy (EM). I-loops are indicated by the yellow rectangles. Insets show 2X enlargements of some of the areas inside the yellow rectangles. b Quantification of i-loop occurrence. Telomere-enriched (telomeric) and non-enriched genomic DNA (bulk) were analyzed in EM as described above. The non-enriched, bulk DNA was digested with KpnI, which generates fragments of 10 kb in average. The fraction of molecules containing one or more i-loops was scored in 3 independent experiments (n = 310, 715, and 845 molecules for telomere-enriched samples and 1466, 776, and 1776 molecules for non-enriched samples). Bars represent the mean with the standard deviation. Single data points are also shown as red dots. P value = 0.0009, was derived from unpaired, two-tailed, Student’s t-test. Source data are provided as a Source Data File. c Size distribution of the i-loops in the telomeric DNA. N = 342 i-loops. Source data are provided as a Source Data File. d Distribution of the number of i-loops per molecule in the telomeric DNA. N = 266 molecules with i-loops. Source data are provided as a Source Data File.
