Fig. 2: RRE-IIB-0/rev complex stoichiometry probed by native MS.
From: Native mass spectrometry reveals the initial binding events of HIV-1 rev to RRE stem II RNA
a ESI spectrum of a 2 µM RRE-IIB-0 RNA and 4 µM rev peptide solution in 9:1 H2O/CH3OH (pH ~7.5, adjusted by addition of ~1.25 mM piperidine; 3 h incubation time) shows signals of free RNA (open circles), 1:1 complex (filled circles), and 1:2 complex (triangles); b stacked area plot illustrating the yield of free RNA (open circles), 1:1 complex (filled circles), and 1:2 complex (triangles), error bars show estimates obtained as described in the Methods section; c Ratio of 1:2 to 1:1 complex yields (diamonds) versus incubation time with error bars as described in the Methods section; d yield of free RNA at t = 1–5 h (gray circles, average values shown as black circles) and e ratio of 1:2 to 1:1 complex yields at t = 1–5 h (gray diamonds, average values shown as black diamonds) versus rev peptide concentration, [rev]0 (RRE-IIB-0 concentration, [RRE]0, was 2 µM in all experiments). Source data are provided as a Source Data file.
