Fig. 3: Relative frequency of DNA bases for each position.

a Relative frequency as specified by design. Note that the peaks in positions 40–50 nt are due to the index. b Measured relative frequencies (n = 1). c Monte-Carlo simulation with deletion error rate λ = 7 (poisson distributed probability), a substitution error rate λ = 2 and an insertion rate λ = 3. The simulated tailing ratio is C:T 5:1. Source data are provided as a Source data file.