Fig. 1: Creating a molecular tag using the Porcupine molecular tagging system.
From: Rapid and robust assembly and decoding of molecular tags with DNA-based nanopore signatures
a Porcupine’s encoding scheme. A digital tag is converted into a codeword to add additional bits for error correction. Each codeword bit is assigned to a unique molbit, where 1s and 0s are represented by the presence or absence of individual molbits in the molecular tag mixture. b A user first defines a digital tag as a binary 96-bit number, and pipettes 1-bits into the molecular tag. The tag is applied to an object, which is then shipped or stored. To read the tag, it is rehydrated and loaded directly onto Oxford Nanopore Technologies’ MinION device. Software then decodes the molecular tag without knowledge of the original digital tag.
