Fig. 4: Effects of the SOST C-tail on LRP6 binding and Wnt1-signaling inhibition.
From: Sclerostin inhibits Wnt signaling through tandem interaction with two LRP6 ectodomains
a The affinities of various SOST mutants for LRP6 E1E2 or LRP6 E1 were measured by MST, and the relative KD values from those measurements are shown. The KD value of SOST WT for LRP6 E1E2 was used as a reference. The experimentally determined KD values are provided in Supplementary Table 2. b The inhibitory effects of SOST mutants on Wnt1 signaling were detected by TopFlash assays. Each luciferase signal observed after co-transfecting plasmids encoding Wnt1 and each SOST mutant was normalized to that found with the empty-vector control. c Differences in the inhibitory activities of SOST with WT LRP6 or the LRP6Δacidic mutant in LRP6-knockout HEK293T cells are shown in the bar graph. Data from three independent experiments (n = 3) were analyzed and expressed as mean ± SEM in b and c.
