Fig. 6: MN toxicity susceptibility marker MMP9 positively correlates with DR6. | Nature Communications

Fig. 6: MN toxicity susceptibility marker MMP9 positively correlates with DR6.

From: Systematic elucidation of neuron-astrocyte interaction in models of amyotrophic lateral sclerosis using multi-modal integrated bioinformatics workflow

Fig. 6

a 20 μm thick unfixed L4/L5 sections of spinal cord from WT mice were labeled with fluorescent probes for DR6, MMP9, and ChAT using RNAscope technique. Representative images are shown. Scale bar: 50 μm. b Pearson’s linear correlation analysis was performed between DR6 and MMP9 in ChAT-positive MNs (r(75) = 0.8693, CI 0.8013–0.9151, two-tailed P < 0.0001). Similar r values were obtained for two additional independent experiments. Representative correlation plot of three experiments repeated independently with similar results. c Mouse WT (blue) or DR6−/− (red) MNs were treated with vehicle, Domoic acid or activating anti-Fas antibody for 7 days. Data are means ± SEM of n = 4 independent experiments and were analyzed by two-way ANOVA (interaction F(2,18) = 0.036, P = 0.96), which revealed no statistical differences for genotypes x treatments. Source data provided as source data file.

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