Fig. 2: Open syntaxin rescues acetylcholine secretion defects in snt-1-null C. elegans.
From: Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans

a Box-and-whisker plots of thrashing assay for N2, snt-1, and open syntaxin; snt-1 double mutants in M9 buffer. snt-1 worms displayed impaired thrashing rates (20.1 thrashes/min), which was increased by the introduction of open syntaxin in the double mutant (48.9 thrashes/min). n = 40 for each strain. In one-way ANOVA statistical tests, F(2,117) = 388 and p = 0.00. Tukey’s test was performed for means analysis in ANOVA. N2 vs. snt-1: **p = 0.00, snt-1 vs. open syntaxin; snt-1: p = 0.00, N2 vs. open syntaxin, snt-1: **p = 0.00. b Aldicarb assays of N2, snt-1, and open syntaxin; snt-1 double mutants. open syntaxin; snt-1 animals displayed slightly increased sensitivity to aldicarb compared to snt-1 single mutants, which were resistant to aldicarb’s paralyzing effects. n = 6. Each assay was conducted with 15–20 worms. Error bars represent SEM. c Representative mPSC traces recorded from snt-1 and open syntaxin; snt-1 worms. d Box-and-whisker plots overlaid with the corresponding data points (squares) of mPSC frequency (left) and amplitude (right) of snt-1 and open syntaxin; snt-1 worms. Two-sample two-sided t-test was performed. **p = 0.00; n.s. p = 0.93. n = 5 for snt-1(md290) and open syntaxin; snt-1(md290) animals. Box-and-whisker plots represent the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers).