Fig. 9: The knock-in open syntaxin mutation enhances exocytosis in GABA receptor mutants.
From: Open syntaxin overcomes exocytosis defects of diverse mutants in C. elegans
a Box-and-whisker plots of thrashing assay for N2, unc-49(e407), and open syntaxin; unc-49(e407) double mutants in M9 buffer. unc-49(e407) worms displayed greatly reduced thrashing rates (5.73 thrashes/min), which was rescued by the introduction of open syntaxin in the double mutant (17.05 thrashes/min). n = 40 for each strain. In one-way ANOVA statistical tests, F(2,117) = 335 and p = 0.00. Tukey’s test was performed for means analysis in ANOVA. For all comparisons, **p = 0.00. b Aldicarb assays of N2, unc-49(e407), and open syntaxin; unc-49(e407) double mutants. unc-49 animals displayed hypersensitivity to aldicarb (gray), which was further enhanced in the double mutant (black). n = 6. Each assay was conducted with 15–20 worms. Error bars represent SEM. c Representative mEPSC traces recorded from N2, unc-49(e407), and open syntaxin; unc-49(e407) worms. d Box-and-whisker plots overlaid with the corresponding data points (squares) of mEPSC frequency (left) and amplitude (right) of N2, unc-49(e407), and open syntaxin; unc-49(e407) worms. Two-sample two-sided t-test, *p = 0.04; n.s. p = 0.75. n = 9 for unc-49(e407) and open syntaxin; unc-49(e407) animals. Box-and-whisker plots represent the median (central line), 25th–75th percentile (bounds of the box), and 5th–95th percentile (whiskers).
