Fig. 2: Reduction in miR398 levels by overexpression of MIR398b and MIR398c genes. | Nature Communications

Fig. 2: Reduction in miR398 levels by overexpression of MIR398b and MIR398c genes.

From: Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance

Fig. 2

a, b GUS signals showing temporal and spatial expression of MIR398b and NAT398b (a) and MIR398c and NAT398c (b). The experiments were repeated at least three times with similar results, and data from one representative experiment are shown. c, e, g Northern blot showing miR398 accumulation in p35S::MIR398b (c), p35S::MIR398c (e), and p35S::MIR398a (g) seedlings. d, f, h Results of qRT-PCR showing relative expression levels of related genes in p35S::MIR398b (d), p35S::MIR398c (f), and p35S::MIR398a (h) seedlings. i miR398 accumulation in tobacco plants transiently expressing MIR398 genes. Error bars are the mean ± SD. Significant differences were determined by one-tailed student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001).

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