Fig. 4: Mechanisms of regulation of pri-miR398b/c by NAT398b/c. | Nature Communications

Fig. 4: Mechanisms of regulation of pri-miR398b/c by NAT398b/c.

From: Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance

Fig. 4

a RNase protection assays showing double-stranded RNA formation between NAT398b/c and pri-miR398b/c. Primers c1, c2, c3, c4, and c5 are located in complementary regions between pri-miRNAs and NAT RNAs; primers i1 and i2 are located in non-complementary regions. b Relative expression levels of MIR398b/c in tobacco plants transiently expressing NAT398b/c and MIR398b/c. c, d Relative RNA levels at different time points after treatment with actinomycin D (100 μg/mL) in Col and p35S::NAT398b (c) and p35S::NAT398c (d) lines. e nat-siR398b-1 accumulation in p35S::NAT398b and p35S::NAT398c seedlings. f Relative expression levels of MIR398b and MIR398c in p35S::nat-siR398b-1 seedlings. Error bars are the mean ± SD. Significant differences were determined by one-tailed student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001).

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