Fig. 6: NAT398b/c attenuation of H2O2 accumulation and thermotolerance in Arabidopsis. | Nature Communications

Fig. 6: NAT398b/c attenuation of H2O2 accumulation and thermotolerance in Arabidopsis.

From: Natural antisense transcripts of MIR398 genes suppress microR398 processing and attenuate plant thermotolerance

Fig. 6

a Northern blot showing miR398 accumulation in seedlings exposed to high temperature for 0 to 1 h. b Relative expression levels of MIR398b, NAT398b, MIR398c, and NAT398c in seedlings exposed to high temperature for 0–1 h. Error bars are the mean ± SD (n = 3 biological replicates). c DAB staining for H2O2 levels in transgenic and mutant plants. d Survival rate of flowers of wild-type, csd1, p35S::amiR-NAT398b, p35S::amiR-NAT398c, p35S::NAT398b, p35S::NAT398c, and CRNAT398b plants under heat stress. e, f Phenotypes of detached leaves (e) and the rate of green leaves (f) of wild-type, csd1, p35S::amiR-NAT398b, p35S::amiR-NAT398c, p35S::NAT398b, p35S::NAT398c, and CRNAT398b plants. Error bars are the mean ± SD (n = 3 biological replicates, a replicate constitutes 60 biologically independent samples for d, and 12 biologically independent samples for f). Significant differences were determined by one-tailed student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001).

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