Fig. 4: Perfusion reduces TEBV EC permeability.
From: Modeling early stage atherosclerosis in a primary human vascular microphysiological system
Permeability of TEBVs to 20 µg/ml of FITC-dextran (500 kDa) (a) and to 10 μg/ml of FITC-IgG (b). The groups are collagen tubing without cells and without perfusion (No cells), TEBVs with cells under static culture of 1 week (Static), TEBVs with cells for 1-week perfusion perfused at 0.5 ml/min per TEBV (0.4 Pa) (perfusion), TEBVs under 1-week perfusion followed by 96 h perfusion with media containing 50 μg/ml eLDL (eLDL), TEBVs following 96 h exposure to 50 μg/ml eLDL followed by 8 days without eLDL (Recovery) (mean ± S.D., n = 3 TEBVs, (a) perfusion: ***P = 0.00066 (10 min), ***P = 0.000105 (20 min), (b) static ***P = < 0.0001 (5 min), **P = 0.00785(10 min), *P = 0.0358 (20 min), Perfusion: ***P < 0.0001 (5 min), **P = 0.00161 (10 min), *P = 0.0488 (20 min), compared to no-cell group by a two-way ANOVA and Tukey post hoc test).
