Fig. 6: Mutational analysis.
From: The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations
a Multiple sequence alignment of conserved helicase motifs (indicated by letters or Roman numerals above the alignment) in human ASCC3, human Brr2 and yeast Slh1p N-terminal cassettes (NC) and C-terminal cassettes (CC). The conserved motif I lysine and motif II aspartate residues of ASCC3 cassettes, which were altered in the present analysis, are highlighted in blue and red, respectively. Color coding of the motifs: involved in ATP binding, blue; involved in RNA binding, yellow; involved in communication of RNA binding and ATPase activities, red. Roles of selected residues are indicated below the alignment. b–d Stopped-flow/fluorescence-based assays monitoring unwinding of 3’-overhang DNA by the indicated ASCC3HR variants using ATP. Schemes below the unwinding traces represent ASCC3HR with altered residues highlighted. WT/K505N/D611A/K1355N/D1453A/KK505/1355NN/DD611/1453AA, ASCC3HR variants as defined in the text.
