Fig. 2: Structural characterization of the stabilized two-helix motif was performed.
From: A protein tertiary structure mimetic modulator of the Hippo signalling pathway
a CD spectra of selected peptides and calculated helicities are shown (for details, see Methods section). Source data are provided as a Source Data file42. b X-ray crystal structure of 4E (orange) co-crystallized with mTEAD4 (grey, PDB: 6SBA) superimposed with the corresponding part of the TEAD-binding motif of VGL4 (blue/yellow, PDB: 4LN0). c 2Fo-Fc electron density map (black, contoured at σ = 1.2) of 4E (PDB: 6SBA) from top and bottom view. d Superimposition of two-helix motif from VGL4 (blue, PDB 4LN0) and peptide 4E (orange, PDB: 6SBA), side chains of residues previously identified as interacting with TEAD are shown explicitly. e Structure of 4E in complex with mTEAD4 (PDB: 6SBA) including all side chains is shown. Hotspot amino acids are highlighted (red) as determined by experimental alanine-scanning using SPR (mTEAD4) as readout. Crystal structure of the E4/mTEAD4 complex is available under PDB: 6SBA.
