Fig. 4: Fitting of the multi-parametric surface plasmon resonance (MP-SPR) experimental data using the Langmuir model.
From: Gamma estimator of Jarzynski equality for recovering binding energies from noisy dynamic data sets
a Kinetic profiles obtained by MP-SPR at four different concentrations, fitted in the full range 600 s. The experimental data is denoted by the circles and the solid lines are the respective fitting curves. b The initial 400 s data at low concentrations used to extract kinetic rate constants. c Observed rate constants (kobs) plotted as a function of the peptide concentration. Linear regression was used to calculate the adsorption (ka) and desorption (kd) rates.
