Fig. 4: In vitro Hydrogen production of the carboxysome shell-based nanoreactor.
From: Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production
a Hydrogen production activity (nmol mg−1 min−1) of isolated free HydA and Shell-HydA at pH 8 as a function of different concentrations of MV+ as the electron mediator reduced by sodium dithionite (DT), fitted with Michaelis-Menten kinetics. Values represent mean ± s.d., n = 3 biologically independent experiments. b Kinetic hydrogen production of free HydA and Shell-HydA using 50 mM DT reduced MV+ as the electron mediator at pH 8. Values represent mean ± s.d., n = 3 biologically independent experiments. c Relative activity of free HydA and Shell-HydA after oxygen exposure for 24 h at 4 °C, as a relative percentage of total activities measured under anaerobic conditions (see also Supplementary Fig. 10). Values represent mean ± s.d., n = 3 biologically independent experiments. ***p = 0.0009 (two-tailed unpaired t-test). Source data are provided as a Source data file.
