Fig. 5: SLG1Ind-complemented plants show strong thermotolerance and high thiolated tRNA level.
From: Natural variations of SLG1 confer high-temperature tolerance in indica rice
a, b Comparison of thermotolerance among IPIC, IPJC, JPIC, and JPJC plants. Three independent homozygous IPIC, IPJC, JPIC, and JPJC lines were selected and treated with 45 °C for 56 h and then recovered under normal condition for 10 days. Data are means ± SD (n = 3 independent transgenic lines. Each transgenic line was analyzed with three biological replicates, with 40–56 seedlings per biological replicate). *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test. Bar = 5 cm. c, d Detection of thiolated tRNAs in slg1, WT, IPIC, and JPJC transgenic lines based on LC/MS-QQQ- (c) and APM- (d) methods. Shoots of 2-leaf-stage seedlings under normal growth conditions were sampled. The loaded amount of tRNA was 3 µg for each lane (d). Data are means ± SD (n = 3 biological replicates). ****P < 0.0001, two-tailed Student’s t-test. The experiment in (d) was repeated three times with similar results. The source data underlying (b–d) are provided as a Source data file.
