Fig. 2: Testing the selective decoupling pulse using real time and constant-time 1H–13C HMQC on MBP.
From: Nearest-neighbor NMR spectroscopy: categorizing spectral peaks by their adjacent nuclei
a Overlay of a section of a real-time 2D 1H–13C methyl HMQC experiment with (orange) and without (black) the selective decoupling pulse on a 500 µM MBP (42.5 kDa) protein. The leucine peaks remain as doublets, whereas the valine peaks collapse into singlets. b Constant-time 1H–13C HMQC experiment with indirect constant-time period set to T = 28 ms (1/1JCC) and shaped pulse applied at time T/2. Valine resonances are positive (blue) whereas leucine peaks are negative (red). c A version of the experiment with constant-time evolution period is set to T = 42 ms (3/(2 1JCC)) completely eliminates the leucine peaks from the spectrum with positive valine peaks remaining (blue). Full spectra are in Supplementary Fig. 5.
