Fig. 2: Targeting of YFP-RAB-A2a at the tips of growing root hairs. | Nature Communications

Fig. 2: Targeting of YFP-RAB-A2a at the tips of growing root hairs.

From: Armadillo-repeat kinesin1 interacts with Arabidopsis atlastin RHD3 to move ER with plus-end of microtubules

Fig. 2

a–d Heatmap of the targeting of YFP-RAB-A2a in a root hair of wild-type (a), rhd3-1 (b), ren9-1 (c), and rhd3-1 ren9-1 (d). e Quantification of swings of YFP-RAB-A2a targeting related to the previous growth direction (marked by white dashed lines in a–d). YFP-RAB-A2a signals are shown in the heatmap format. The color code illustrates the signal intensity. Red arrows indicate the targeting direction of YFP-RAB-A2a at the tips. White dashed lines present the previous direction of root-hair growth. The experiment was independently repeated six times. In wild-type, there were rapid swings of YFP-RAB-A2a (a, e); in rhd3-1, rapid swings were suspected from 18′ to 39′ (b, e) at which the root hair started to bend. In ren9-1, there were also suspensions of rapid swings at 7.5′-9′and 11′-15.5′ (c, e). In rhd3-1 ren9-1, there was no swing at all; YFP-RAB-A2a was targeted toward the bending direction (d, e). The scale bars = 10 µm.

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