Fig. 8: ARK1, but not ARK1ΔARM interacts with RHD3. | Nature Communications

Fig. 8: ARK1, but not ARK1ΔARM interacts with RHD3.

From: Armadillo-repeat kinesin1 interacts with Arabidopsis atlastin RHD3 to move ER with plus-end of microtubules

Fig. 8

a Co-purification of mCherry-RHD3 with ARK1–GFP (line 3) but not with ARK1ΔARM (line 2) or GFP (line 1) using GFP-trap. mCherry-RHD3 was detected by anti-RFP; ARK1–GFP, ARK1ΔARM, or GFP was detected by anti-GFP. Input is a total protein extract samples; IP is purified samples with GFP-trap. b Venus-based Bi-molecular fluorescence complementation (BiFC) analyses of nVenus-RHD3 and ARK1-cVenus or ARK1ΔARM-cVenus or ARM-cVenus. mCherry-HDEL from the same gene cassette was used as an expression indicator. This experiment was independently repeated three times. Scale bars = 10 μm. Source Data are available.

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