Fig. 1: Cas9 diversity and characterization approach. | Nature Communications

Fig. 1: Cas9 diversity and characterization approach.

From: A catalogue of biochemically diverse CRISPR-Cas9 orthologs

Fig. 1

a Phylogenetic representation of the diversity provided by Cas9 orthologs. Type II-A, B, and C systems are color-coded, red, blue, and green, respectively. Distinct phylogenetic clades are numbered I–X. Those selected for the study are indicated with a black dot. Cas9s whose structure has been determined are also designated. b Biochemical approach used to directly capture target cleavage and assess protospacer adjacent motif (PAM) recognition. Experiments were assembled using Cas9 protein produced by IVT.

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