Fig. 5: Essential genes in RGP—NMV_1479 and NMV_0559—are conditionally essential.

a Gene organisation of the putative antitoxin NMV_1478 (highlighted in red) with its neighbouring NMV_1479 toxin. Results of the mutagenesis (represented by scissors) are shown. Viable mutant (represented by a grey diplococcus); lethal phenotype (represented by a skull). b Gene organisation of the tps RGP (RGP_0) to which NMV_0559 (highlighted in red) belongs (Supplementary Data 9). Genes on the + strand are in white, genes on the—strand are in black. Results of the mutagenesis (scissors) are shown. Viable mutant (grey diplococcus); lethal phenotype (skull). In contrast to NMV_0559, each of the other target genes in the tps RGP could be mutated individually (not shown for readability).