Fig. 6: NMV_1317 encodes a novel histidinol-phosphatase. | Nature Communications

Fig. 6: NMV_1317 encodes a novel histidinol-phosphatase.

From: Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen

Fig. 6

a Histidine biosynthesis pathway in the meningococcus, with genes highlighted in red. PRPP 5-phosphoribosyl diphosphate, PRFAR phosphoribulosylformimino-AICAR-P, IGP erythro-imidazole-glycerol-P. b Growth on M9 minimal medium, with or without added histidine (His). The plates also contained 0.5 mM IPTG for inducing expression of the complementing genes. WT, strain 8013; Δ1317, ΔNMV_1317 mutant; Δ1317::1317, ΔNMV_1317 complemented with NMV_1317; Δ1317::hisBEC, ΔNMV_1317 cross-complemented with hisBEC from E. coli, which encodes the unrelated histidinol-phosphatase present in this species; Δ1718, ΔNMV_1718 mutant; Δ1718::hisBEC, control showing that ΔNMV_1718 (hisH) cannot be cross-complemented by hisBEC. Source data are provided as a Source Data file.

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