Fig. 7: Assaying piliation in the mutants in genes not previously associated with T4P biology in N. meningitidis. | Nature Communications

Fig. 7: Assaying piliation in the mutants in genes not previously associated with T4P biology in N. meningitidis.

From: Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen

Fig. 7

The WT strain and a non-piliated ΔpilD mutant were included as positive and negative controls, respectively. ΔtsaP, ΔtsaP mutant; Δ1205, ΔNMV_1205 mutant; Δ2228, ΔNMV_2228 mutant. a T4P purified using a shearing/precipitation method were separated by SDS-PAGE and either stained with Coomassie blue (upper panel) or analysed by immunoblotting using an antibody against the major pilin PilE (lower panel)59. Samples were prepared from equivalent numbers of cells and identical volumes were loaded in each lane. MW molecular weight marker lane, with values in kDa. Source data are provided as a Source Data file. b T4P were quantified by whole-cell ELISA using a monoclonal antibody specific for the filaments of strain 801358. Equivalent numbers of cells, based on OD600 readings, were applied to the wells of microtiter plates. Results are expressed in % piliation (ratio to WT) and are the average ± standard deviations from five independent experiments. Source data are provided as a Source Data file. For statistical analysis, one-way ANOVA followed by Dunnett’s multiple comparison tests were performed (****P < 0.0001).

Back to article page