Fig. 8: Functional analysis of the mutants in genes not previously associated with T4P biology in N. meningitidis. | Nature Communications

Fig. 8: Functional analysis of the mutants in genes not previously associated with T4P biology in N. meningitidis.

From: Construction of a complete set of Neisseria meningitidis mutants and its use for the phenotypic profiling of this human pathogen

Fig. 8

The WT strain and a non-piliated pilD mutant were included as positive and negative controls, respectively. a Aggregation in liquid culture as assessed by phase-contrast microscopy38. ΔtsaP::tsaP, ΔtsaP complemented with tsaP; ΔtsaP/ΔpilT, double mutant in tsaP and pilT; Δ1025::1205, ΔNMV_1205 complemented with NMV_1205; Δ1205/ΔpilT, double mutant in NMV_1205 and pilT; Δ2228::2228, ΔNMV_2228 complemented with NMV_2228. Scale bar, 20 µm. Source data are provided as a Source Data file. b Quantification of the competence for DNA transformation. Equivalent numbers of recipient cells were transformed using a rpoB PCR product containing a point mutation leading to rifampicin resistance. Results are expressed as transformation frequencies and are the average ± standard deviations from four independent experiments. Source data are provided as a Source Data file. For statistical analysis, one-way ANOVA followed by Dunnett’s multiple comparison tests were performed (****P < 0.0001).

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