Fig. 2: DILA1 promotes cell proliferation and tamoxifen resistance.
From: LncRNA DILA1 inhibits Cyclin D1 degradation and contributes to tamoxifen resistance in breast cancer
a–d MCF-Re cells were transfected with NC or ASOs targeting DILA1 and then treated with 3 μM tamoxifen (Tam). a MTT assay showing relative cell growth at 0, 24, 48, and 72 h. Representative images of colony formation (b) or EdU incorporation by fluorescence microscopy (c). Flow cytometry showing the cell cycle distribution of cells (d). e–h MCF7-Pa cells were transfected with control vector or vector expressing DILA1 and then treated with 3 μM tamoxifen (Tam). MTT assay showing relative cell growth at 0, 24, 48, and 72 h (e). Representative images of colony formation (f) or EdU incorporation by fluorescence microscopy (g). Flow cytometry showing the cell cycle distribution of cells (h). For c, g, scale bars represented 100 μm. For all experiments, n = 3 biologically independent experiments. For a–h, means ± s.d. were shown, and p values were determined by two-tailed Student’s test.
