Fig. 1: ES cells self-organisation in 3D in vitro model of early epiblast morphogenesis. | Nature Communications

Fig. 1: ES cells self-organisation in 3D in vitro model of early epiblast morphogenesis.

From: Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause

Fig. 1

a E4.5 blastocyst and E5.5 egg cylinder-stage embryos stained for the epithelial polarity marker Par6. The apical domain (arrow) in the E5.5 epiblast surrounds the proamniotic cavity. The epiblast is marked by Oct4 (top panel). The naive pre-implantation epiblast is marked by Nanog (bottom panel), and the nuclei are counterstained with DAPI. b Establishment of epithelial rosettes in 3D ES cell culture. Wild-type E14 ES cells were cultured in N2B27 medium supplemented with DMSO (control) or 2i for 24, 36 and 48 h. Data represent mean ± SD, three independent experiments. c Wild-type E14 ES cells grown in 3D culture conditions in the presence of DMSO or 2i for 24, 36 and 48 h and stained for Par6, Oct4 and DAPI. The apical domain surrounding the lumen is marked by an arrow. d Wild-type E14 ES cells grown in 3D culture conditions in the presence of DMSO or 2i for 24, 36 and 48 h and stained for Par6, Nanog and DAPI. Scale bars, 10 µm.

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