Fig. 8: Wnt signalling is activated in an autocrine manner during diapause. | Nature Communications

Fig. 8: Wnt signalling is activated in an autocrine manner during diapause.

From: Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause

Fig. 8

a Control TCF/Lef:H2B-GFP Wls +/+ and TCF/Lef:H2B-GFP Wls Δ/Δ embryos isolated at EDG7.5 and stained for GFP, Nanog and DAPI. Note the lack of GFP expression in the epiblast of Wls-deficient blastocysts. b Percentage of GFP-positive cells in epiblast in control TCF/Lef:H2B-GFP Wls (+/+ and +/Δ) and TCF/Lef:H2B-GFP Wls Δ/Δ embryos at EDG7.5. Data represent mean ± SEM, n = number of embryos, two-tailed unpaired Student’s t test, ***P < 0.001. c Wls control (+/Δ) and knockout (Δ/Δ) embryos stained for Esrrb, Par6 and DAPI. d Esrrb expression in the epiblast cells of Wls control (+/+ and +/Δ) and knockout (Δ/Δ) embryos at EDG7.5. Data represent mean ± SEM, n = cells measured in eight Wls control and five knockout embryos, two-tailed unpaired Student’s t test, the exact P value is noted in the figure. e Wls control (+/Δ) and knockout (Δ/Δ) embryos stained for Nanog, Par6 and DAPI. f The number of epiblast cells in Wls control (+/+ and +/Δ) and knockout (Δ/Δ) embryos at EDG7.5 based on Nanog expression. Data represent mean ± SEM, n = number of embryos, two-tailed unpaired Student’s t test, the exact P value is displayed in the figure. g Function of the Wnt/β-catenin/Esrrb cascade in vitro and in vivo. Scale bars, 10 µm.

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