Fig. 1: Insulin circular intronic RNAs are detectable in mouse, rat, and human islets.
From: A circular RNA generated from an intron of the insulin gene controls insulin secretion
a Agarose gel electrophoresis picture showing circRNAs amplified by qPCR using divergent primers targeting the intron 2 of mouse Ins2, rat Ins2, and human INS. b Loci of sequenced qPCR products of mouse Ins2 (mm10), rat Ins2 (rn6), and human INS (hg38) intron 2 lariat and full-length circRNAs. The image was obtained using the UCSC genome browser (http://genome.ucsc.edu). c Agarose gel electrophoresis picture of lariat mouse and rat ci-Ins2 and human ci-INS qPCR products amplified with over-junction primers. a, c cDNA synthesis of islet RNA was performed with (+) or without (−) DNase treatment and with (+) or without (−) addition of reverse transcriptase (RT). n = 1 independent experiment. Source data are provided as a Source Data file.
