Fig. 4: ci-Ins2 is involved in rat β-cell secretory activity.

a–d Dissociated rat islet cells were transfected with control (black circles) or ci-Ins2 (clear circles) gapmeRs. Experiments were performed 48 h after transfection. a ci-Ins2 and Ins2 normalized to Hprt1, measured by qPCR, and expressed relative to control. n = 6 independent experiments. ***p < 0.0001 by two-tailed one-sample t-test. b Insulin secretion in response to 2 mM glucose (G), 20 mM G, or 30 mM KCl represented as the fold change vs. control basal (2 mM G) condition. n = 5 independent experiments. *p = 0.018 and **p = 0.0007 as indicated, and ^#p = 0.016, ^###p < 0.0001 (20 mM G) and ^###p = 0.0004 (30 mM KCl) vs. 2 mM G control by one-way ANOVA and Tukey post hoc test. c Basal and 48 h prolactin-stimulated β-cell proliferation determined by Ki67 and insulin staining. n = 9 independent experiments. ^###p = 0.00005 vs. basal control condition by one-way ANOVA and Tukey post hoc test. d Basal and 24 h cytokine-induced apoptosis determined by scoring pycnotic nuclei. n = 6 independent experiments. ^###p = 0.0002 vs. basal control condition by one-way ANOVA and Games-Howell post hoc test. a–d Data and mean are represented ± s.d. Source data are provided as a Source Data file.