Fig. 2: Investigating cross-interactions between different QS systems. | Nature Communications

Fig. 2: Investigating cross-interactions between different QS systems.

From: Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states

Fig. 2

a Heat map of induction ratio from 84 combinations of different QS components. All possible 84 response promoter/signal inducer/actuator combinations were created. The induction ratio was defined as dividing the normalized GFP fluorescence from different combinations by the control strain harboring only response promoter and two corresponding empty plasmids. b Evaluating cross-talk between response promoter from E. faecalis (PprgQ) and regulator LuxR. c Evaluating cross-talk between response promoter from E. faecalis (PprgQ) and regulator LasR. d Evaluating cross-talk between response promoter from E. faecalis (PprgQ) and regulator AgrAC. Each QS component was expressed by constitutive trc promoter. Pre-cultured recombinant strains were grown in 25 mL of MOPS medium at 30 °C with 220 rpm orbital shaking. Cell fluorescence and cell density (OD600) were measured after 30 h of culture on a Cytation 3 imaging reader system (BioTek, Winooski, USA). All fluorescence measurements were normalized by dividing measured fluorescence values by the OD600 of that well to conduct a per-cell measurement. Values are shown as mean ± SD (n = 3 biological replicates). Source data are available in the Source data file.

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