Fig. 6: Enhancing metabolic production yields by resource allocator circuits. | Nature Communications

Fig. 6: Enhancing metabolic production yields by resource allocator circuits.

From: Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states

Fig. 6

a Schematic of r-BOX cycle. b The r-BOX cycle was driven by different triggering times of QVX circuits. Pi (i = 1–6) indicated using Pi (i = 1–6) promoters for LuxI and LuxR expression. (B1) PluxI I11 response promoters fused with each pathway enzyme were placed on pCOLADuet-1. (B2) PluxI I11 response promoters fused with each pathway enzyme were placed on pETDuet-1. (B3) PluxI I11 was replaced with native PluxI promoter. c The effect of different triggering times of QEX circuits driving MazF expression on MCFA production. Pi (i = 1–6) indicated using Pi (i = 1–6) promoters for CcfA expression. d The effect of protecting host factors from MazF on MCFA titers. Pre-cultured recombinant strains were grown in 25 mL of MOPS medium at 30 °C with 220 rpm orbital shaking. MCFA titers and cell density (OD600) were measured after 48 h of culture. Values are shown as mean ± SD (n = 3 biological replicates). Source data are available in the Source data file.

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